Hotspot

trnW-CCA-trnP-UGG

LSC · 64585-64750 · 166 bp

Back to Drymaria

Pi 0.0897

MarkerSeek score 83.50

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 14
pi: 0.0897
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	253-257 bp	1.0	86.1
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	245-249 bp	1.0	85.8
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	252-256 bp	1.0	85.7
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	280-284 bp	1.0	85.2
trnW-CCA-trnP-UGG_p5	AACAAGAACGGAATCACGCT	CTTGGTAGCGCGTTTGTTTT	45.0	45.0	58.1	57.9	313-317 bp	1.0	84.2