Hotspot

ndhC-trnV-UAC

LSC · 48916-49955 · 1040 bp

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Pi 0.103

MarkerSeek score 85.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1040
variable_sites: 101
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCCAGAAAATATCATATTCA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.3	58.7	1086-1097 bp	1.0	51.4
ndhC-trnV-UAC_p2	TGCCCAGAAAATATCATATTCA	TTCGAGTCCGTATAGCCCTA	31.8	50.0	53.3	57.1	1085-1096 bp	1.0	51.4
ndhC-trnV-UAC_p3	ATGCCCAGAAAATATCATATTCA	GTTCGAGTCCGTATAGCCCT	30.4	55.0	53.9	58.7	1087-1098 bp	1.0	51.4
ndhC-trnV-UAC_p4	TGCCCAGAAAATATCATATTCAT	GTTCGAGTCCGTATAGCCCT	30.4	55.0	53.9	58.7	1086-1097 bp	1.0	51.4
ndhC-trnV-UAC_p5	AATGCCCAGAAAATATCATATTCA	GTTCGAGTCCGTATAGCCCT	29.2	55.0	54.7	58.7	1088-1099 bp	1.0	51.4