Hotspot

trnT-UGU-trnL-UAA

LSC · 44974-45791 · 818 bp

Back to Drymaria

Pi 0.128

MarkerSeek score 84.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 97
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	TCCAGGAATAGTTGGAGCCA	AAAAAGGGAGTTGGCTCAGG	50.0	50.0	58.0	58.0	1622 bp	0.5	59.7
trnT-UGU-trnL-UAA_p2	TCCAGGAATAGTTGGAGCCA	ACAGCACAATCAACCCCATT	50.0	45.0	58.0	58.0	1686 bp	0.5	59.6
trnT-UGU-trnL-UAA_p3	ATCTTGGGGTTTGCAACGAT	AAAAAGGGAGTTGGCTCAGG	45.0	50.0	58.1	58.0	1706 bp	0.5	59.4
trnT-UGU-trnL-UAA_p4	ATCTTGGGGTTTGCAACGAT	ACAGCACAATCAACCCCATT	45.0	45.0	58.1	58.0	1770 bp	0.5	59.3
trnT-UGU-trnL-UAA_p5	CTAGACCCGTTGACCCTTTG	AAAAAGGGAGTTGGCTCAGG	55.0	50.0	57.9	58.0	1548 bp	0.5	59.2