Hotspot

petN-psbM

LSC · 27518-28099 · 582 bp

Back to Drymaria

Pi 0.0879

MarkerSeek score 81.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 50
pi: 0.0879
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGAAGTGGACT	AAAACGGTCAGCCAAAATGA	47.6	40.0	57.8	56.4	631-661 bp	1.0	65.7
petN-psbM_p2	AGTATGGGGAAGAAGTGGACT	AAACGGTCAGCCAAAATGAT	47.6	40.0	57.8	56.2	630-660 bp	1.0	63.9
petN-psbM_p3	AGTATGGGGAAGAAGTGGACT	AACGGTCAGCCAAAATGATT	47.6	40.0	57.8	56.2	629-659 bp	1.0	63.9
petN-psbM_p4	AGTATGGGGAAGAAGTGGACT	AAAAACGGTCAGCCAAAATGA	47.6	38.1	57.8	57.2	632-662 bp	1.0	63.7
petN-psbM_p5	AGTATGGGGAAGAAGTGGACT	TGTAAAAACGGTCAGCCAAA	47.6	40.0	57.8	56.1	635-665 bp	1.0	62.8