Hotspot

rpoB-trnC-GCA

LSC · 26006-26762 · 757 bp

Back to Drymaria

Pi 0.103

MarkerSeek score 80.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 77
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	CCATTCTTGACCTGCGGTTA	50.0	50.0	58.0	57.9	1749-1858 bp	1.0	85.9
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1332-1424 bp	1.0	85.1
rpoB-trnC-GCA_p3	CCGGGGCTTTGCAATATTTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1212-1304 bp	1.0	85.1
rpoB-trnC-GCA_p4	CTCCCTGTCGGTTTTCCAAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1099 bp	0.5	59.1
rpoB-trnC-GCA_p5	CTCCCTGTCGGTTTTCCAAA	CCATTCTTGACCTGCGGTTA	50.0	50.0	58.0	57.9	1516 bp	0.5	58.9