Hotspot

matK-trnK-UUU

LSC · 3446-4178 · 733 bp

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Pi 0.0767
MarkerSeek score 82.80
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
733
variable_sites
54
pi
0.0767
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TGTTGCCAAGATCTTTCTAGTTCT AACGGTAGAGTACTCGGCTT 37.5 50.0 58.1 58.2 785-804 bp 1.0 54.0
matK-trnK-UUU_p2 TGTTGCCAAGATCTTTCTAGTTCT ACGGTAGAGTACTCGGCTTT 37.5 50.0 58.1 58.2 784-803 bp 1.0 54.0
matK-trnK-UUU_p3 TGTTGCCAAGATCTTTCTAGT AACGGTAGAGTACTCGGCTT 38.1 50.0 54.9 58.2 785-804 bp 1.0 53.8
matK-trnK-UUU_p4 TGTTGCCAAGATCTTTCTAGT ACGGTAGAGTACTCGGCTTT 38.1 50.0 54.9 58.2 784-803 bp 1.0 53.8
matK-trnK-UUU_p5 TGTTGCCAAGATCTTTCTAGTTC ACGGTAGAGTACTCGGCTTT 39.1 50.0 56.8 58.2 784-803 bp 1.0 53.4