Pi
0.00079
MarkerSeek score
57.80
Primer available
yes
Label
ndhA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhA_p1 | CAACCCAGTTACCATAGAGAACA | CCGCCGGGTTTTATCAATTT | 43.5 | 45.0 | 57.8 | 57.0 | 2503-2509 bp | 1.0 | 47.3 |
| ndhA_p2 | CAACCCAGTTACCATAGAGAACA | GCCGGGTTTTATCAATTTGCA | 43.5 | 42.9 | 57.8 | 58.0 | 2501-2507 bp | 1.0 | 46.6 |
| ndhA_p3 | ACCCAGTTACCATAGAGAACA | CCGCCGGGTTTTATCAATTT | 42.9 | 45.0 | 55.7 | 57.0 | 2501-2507 bp | 1.0 | 46.2 |
| ndhA_p4 | ACCCAGTTACCATAGAGAACA | GCCGGGTTTTATCAATTTGCA | 42.9 | 42.9 | 55.7 | 58.0 | 2499-2505 bp | 1.0 | 45.5 |
| ndhA_p5 | TCAACCCAGTTACCATAGAGAAC | CCGCCGGGTTTTATCAATTT | 43.5 | 45.0 | 57.6 | 57.0 | 2504-2510 bp | 1.0 | 45.2 |