Hotspot

ndhF-rpl32

SSC · 115706-116637 · 932 bp

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Pi 0.00665
MarkerSeek score 50.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
932
variable_sites
13
pi
0.00665
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ATTGGGCGGATTTAGCAACT AGGGCGTAGGGTATCCATTT 45.0 50.0 57.9 58.2 2284-2299 bp 1.0 78.5
ndhF-rpl32_p2 GGGGAAATTGGGCGGATTTA AGGGCGTAGGGTATCCATTT 50.0 50.0 58.2 58.2 2290-2305 bp 1.0 77.9
ndhF-rpl32_p3 TTTTTGACAAGCATTCGCCG AGGGCGTAGGGTATCCATTT 45.0 50.0 58.2 58.2 2085-2100 bp 1.0 77.9
ndhF-rpl32_p4 CCCACATACGGCGAAGATTT AGGGCGTAGGGTATCCATTT 50.0 50.0 58.3 58.2 1675-1690 bp 1.0 77.4
ndhF-rpl32_p5 ATTGGGCGGATTTAGCAACT AAAAAGCTTTCAACGCTGCC 45.0 45.0 57.9 58.4 1756-1760 bp 1.0 76.3