Hotspot

psaJ-rpl33

LSC · 69764-70250 · 487 bp

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Pi 0.000821
MarkerSeek score 57.00
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
487
variable_sites
1
pi
0.000821
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ACATATCTTTCCGTGGCACC AACATCTTTACCCTTGGCCA 50.0 45.0 58.0 57.0 627-632 bp 1.0 72.5
psaJ-rpl33_p2 ACATATCTTTCCGTGGCACC CACGATTTCGAACACAACTGG 50.0 47.6 58.0 58.1 673-678 bp 1.0 71.6
psaJ-rpl33_p3 TCCGGATGCGTTGATATTCC AACATCTTTACCCTTGGCCA 50.0 45.0 57.8 57.0 544-549 bp 1.0 71.6
psaJ-rpl33_p4 ACATATCTTTCCGTGGCACC TGGAAATGCCCATTGATTCCT 50.0 42.9 58.0 57.9 700-705 bp 1.0 71.4
psaJ-rpl33_p5 GTTCGGGTCTTTAGCAGGTT AACATCTTTACCCTTGGCCA 50.0 45.0 57.8 57.0 589-594 bp 1.0 71.4