Hotspot

petA-psbJ

LSC · 65451-66324 · 874 bp

Back to Dryas

Pi 0.000917
MarkerSeek score 60.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
874
variable_sites
2
pi
0.000917
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC TCCGGATTAGGTTCATCCCT 50.0 50.0 58.0 57.5 1013-1017 bp 1.0 76.3
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC CCGGATTAGGTTCATCCCTG 50.0 55.0 58.0 57.4 1012-1016 bp 1.0 75.8
petA-psbJ_p3 CATTACGTGTCCAAGGCCTT TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1010-1014 bp 1.0 75.2
petA-psbJ_p4 CATTACGTGTCCAAGGCCTT CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1009-1013 bp 1.0 74.7
petA-psbJ_p5 TTACGTGTCCAAGGCCTTTT TCCGGATTAGGTTCATCCCT 45.0 50.0 57.6 57.5 1008-1012 bp 1.0 73.8