Hotspot

trnT-GGU-psbD

LSC · 33243-34671 · 1429 bp

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Pi 0.00207
MarkerSeek score 65.30
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1429
variable_sites
5
pi
0.00207
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1539-1593 bp 1.0 83.2
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1542-1596 bp 1.0 83.0
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1541-1595 bp 1.0 83.0
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 1544-1598 bp 1.0 82.8
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGCCAGACCAACCTACAAAA 50.0 50.0 58.3 58.0 1516-1570 bp 1.0 81.5