Hotspot

trnE-UUC-trnT-GGU

LSC · 32664-33169 · 506 bp

Back to Dryas

Pi 0.00356
MarkerSeek score 52.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
506
variable_sites
4
pi
0.00356
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 638-648 bp 1.0 81.3
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 617-627 bp 1.0 80.9
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 614-624 bp 1.0 80.8
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 600-610 bp 1.0 80.6
trnE-UUC-trnT-GGU_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 579-589 bp 1.0 80.6