Hotspot

trnK-UUU-rps16

LSC · 4199-5187 · 989 bp

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Pi 0.00121
MarkerSeek score 61.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
989
variable_sites
3
pi
0.00121
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1111-1122 bp 1.0 79.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1110-1121 bp 1.0 79.5
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTTTTACGGAGCTTCGCCTT 50.0 45.0 58.2 58.1 1057-1068 bp 1.0 78.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTTTTACGGAGCTTCGCCTT 50.0 45.0 58.2 58.1 1056-1067 bp 1.0 78.9
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 1120-1131 bp 1.0 78.6