Hotspot

trnN-GUU-rpl32

SSC · 111038-115296 · 4259 bp

Back to Drummondita

Pi 0.0036
MarkerSeek score 47.10
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
4259
variable_sites
23
pi
0.0036
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 GCTTTTGCTAATCATAGTTATTTTGTG AGTGTAAAGTGTAATTAGGCA 29.6 33.3 56.6 52.5 213-217 bp 0.666667 27.8
trnN-GUU-rpl32_p2 GCTTTTGCTAATCATAGTTATTTTGTG AGTGTAAAGTGTAATTAGGCAA 29.6 31.8 56.6 53.4 213-217 bp 0.666667 27.8
trnN-GUU-rpl32_p3 GCTTTTGCTAATCATAGTTATTTTGT AGTGTAAAGTGTAATTAGGCA 26.9 33.3 55.4 52.5 213-217 bp 0.666667 27.8
trnN-GUU-rpl32_p4 GCTTTTGCTAATCATAGTTATTTTGT AGTGTAAAGTGTAATTAGGCAA 26.9 31.8 55.4 53.4 213-217 bp 0.666667 27.8
trnN-GUU-rpl32_p5 GCTTTTGCTAATCATAGTTATTTTGTG AGTGTAAAGTGTAATTAGGCAAT 29.6 30.4 56.6 54.0 213-217 bp 0.666667 27.8