Hotspot

psbE-petL

LSC · 66296-67665 · 1370 bp

Back to Drummondita

Pi 0.00438

MarkerSeek score 57.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1370
variable_sites: 9
pi: 0.00438
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCTGCTAGGAGAAAACCA	50.0	45.0	57.9	56.7	1506-1517 bp	1.0	71.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCTGCTAGGAGAAAACCAA	50.0	45.0	57.9	56.7	1505-1516 bp	1.0	71.0
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAGCTGCTAGGAGAAAACCA	45.0	45.0	58.2	56.7	1515-1526 bp	1.0	70.0
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AGCTGCTAGGAGAAAACCAA	45.0	45.0	58.2	56.7	1514-1525 bp	1.0	70.0
psbE-petL_p5	TGCTGACGAATAACCAACCC	AGCTGCTAGGAGAAAACCAA	50.0	45.0	58.2	56.7	1519-1530 bp	1.0	69.9