Hotspot

rbcL-accD

LSC · 58111-58667 · 557 bp

Back to Drummondita

Pi 0.00598

MarkerSeek score 61.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 5
pi: 0.00598
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	AGGACCAAGACTATCCATTGA	55.0	42.9	58.0	55.8	743-746 bp	1.0	54.3
rbcL-accD_p2	GGAGGCTAGCAAATGGAGTC	AGGACCAAGACTATCCATTGA	55.0	42.9	58.0	55.8	744-747 bp	1.0	54.3
rbcL-accD_p3	TGCTCGCGAAGGTAATGAAA	AGGACCAAGACTATCCATTGA	45.0	42.9	57.9	55.8	771-774 bp	1.0	53.9
rbcL-accD_p4	TTGCTCGCGAAGGTAATGAA	AGGACCAAGACTATCCATTGA	45.0	42.9	57.9	55.8	772-775 bp	1.0	53.9
rbcL-accD_p5	AAATGGAGTCCTGAACTGGC	AGGACCAAGACTATCCATTGA	50.0	42.9	57.8	55.8	734-737 bp	1.0	52.9