Hotspot

atpF

LSC · 11745-13116 · 1372 bp

Back to Driessenia

Pi 0.0112

MarkerSeek score 54.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1372
variable_sites: 23
pi: 0.0112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GCCTACTTGCAGGACAGTAC	TTAGCTGTCGGACTTGCTTC	55.0	50.0	58.0	57.9	2248-2264 bp	1.0	81.3
atpF_p2	GCCTACTTGCAGGACAGTAC	TGATTTCTGCCGCTTCTGTT	55.0	45.0	58.0	58.1	2280-2296 bp	1.0	81.1
atpF_p3	GTATCATCAAACCGTCCCCC	TTAGCTGTCGGACTTGCTTC	55.0	50.0	58.0	57.9	2390-2406 bp	1.0	81.0
atpF_p4	GAAATCTCACCCCGACCATC	TTAGCTGTCGGACTTGCTTC	55.0	50.0	58.0	57.9	2508-2524 bp	1.0	80.8
atpF_p5	GTATCATCAAACCGTCCCCC	TGATTTCTGCCGCTTCTGTT	55.0	45.0	58.0	58.1	2422-2438 bp	1.0	80.8