Hotspot

ndhB-trnL-CAA

IRa · 146307-146919 · 613 bp

Back to Driessenia

Pi 0.00745
MarkerSeek score 67.80
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
IRa
length_bp
613
variable_sites
6
pi
0.00745
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 CCAGGAATATCAATGAACCCGA GTCTCGCGTGTCTACCATTT 45.5 50.0 57.9 58.0 662-699 bp 1.0 68.0
ndhB-trnL-CAA_p2 CCAGGAATATCAATGAACCCGA CTCGCGTGTCTACCATTTCA 45.5 50.0 57.9 58.0 660-697 bp 1.0 68.0
ndhB-trnL-CAA_p3 TCAATTGCTCAGGATACCCT GTCTCGCGTGTCTACCATTT 45.0 50.0 55.9 58.0 635-672 bp 1.0 67.8
ndhB-trnL-CAA_p4 TCAATTGCTCAGGATACCCT CTCGCGTGTCTACCATTTCA 45.0 50.0 55.9 58.0 633-670 bp 1.0 67.8
ndhB-trnL-CAA_p5 CCAGGAATATCAATGAACCCGA TACCATTTCACCACCAAGGC 45.5 50.0 57.9 58.1 650-687 bp 1.0 67.4