Hotspot

rps15-ycf1

SSC · 125845-126195 · 351 bp

Back to Driessenia

Pi 0.0533

MarkerSeek score 77.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 351
variable_sites: 25
pi: 0.0533
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCCGATTTTCTTCTTCTGA	TGTATATATGTATCCCCCGTTGA	42.9	39.1	56.7	56.1	418-425 bp	1.0	47.2
rps15-ycf1_p2	TCCCCGATTTTCTTCTTCTGAA	TGTATATATGTATCCCCCGTTGA	40.9	39.1	57.4	56.1	418-425 bp	1.0	47.2
rps15-ycf1_p3	TCCCCGATTTTCTTCTTCTGA	TGTATATATGTATCCCCCGTTG	42.9	40.9	56.7	54.6	418-425 bp	1.0	47.2
rps15-ycf1_p4	ATCCCCGATTTTCTTCTTCTGA	TGTATATATGTATCCCCCGTTGA	40.9	39.1	57.2	56.1	419-426 bp	1.0	47.2
rps15-ycf1_p5	TCCCCGATTTTCTTCTTCTGA	ATATATGTATCCCCCGTTGA	42.9	40.0	56.7	52.6	415-422 bp	1.0	47.2