Hotspot

rpoA-rps11

LSC · 80907-80971 · 65 bp

Back to Driessenia

Pi 0.041

MarkerSeek score 69.30

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 4
pi: 0.041
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	AACACACTTCCACCGTAGTG	CGTGATGTAACCCCTATGCC	50.0	55.0	57.8	58.1	179 bp	1.0	78.9
rpoA-rps11_p2	CAACACACTTCCACCGTAGT	CGTGATGTAACCCCTATGCC	50.0	55.0	57.8	58.1	180 bp	1.0	78.9
rpoA-rps11_p3	CACCGTAGTGTCCGAGTAGA	CGTGATGTAACCCCTATGCC	55.0	55.0	58.3	58.1	169 bp	1.0	78.8
rpoA-rps11_p4	AACACACTTCCACCGTAGTG	ATGTAACCCCTATGCCCCAT	50.0	50.0	57.8	58.2	175 bp	1.0	78.7
rpoA-rps11_p5	CAACACACTTCCACCGTAGT	ATGTAACCCCTATGCCCCAT	50.0	50.0	57.8	58.2	176 bp	1.0	78.7