Hotspot

psbT-pbf1

LSC · 76083-76154 · 72 bp

Back to Driessenia

Pi 0.102

MarkerSeek score 73.20

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 11
pi: 0.102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	TTCTTCGGACACATTTGGCA	ATCCATTCGAAGAACACGGG	45.0	50.0	58.0	58.0	533 bp	0.666667	68.5
psbT-pbf1_p2	GCTGGTATTGACCCGGATTT	ATCCATTCGAAGAACACGGG	50.0	50.0	57.9	58.0	482 bp	0.666667	68.5
psbT-pbf1_p3	ACACATTTGGCATGGTGCTA	ATCCATTCGAAGAACACGGG	45.0	50.0	58.1	58.0	525 bp	0.666667	68.1
psbT-pbf1_p4	ATTGTTAGGGCCGATGTTCC	ATCCATTCGAAGAACACGGG	50.0	50.0	57.9	58.0	794 bp	0.666667	67.9
psbT-pbf1_p5	GGAATTGTTAGGGCCGATGT	ATCCATTCGAAGAACACGGG	50.0	50.0	57.9	58.0	797 bp	0.666667	67.9