Hotspot

psbB-psbT

LSC · 75789-75974 · 186 bp

Back to Driessenia

Pi 0.0179

MarkerSeek score 55.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 5
pi: 0.0179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCTGGTATTGACCCGGATTT	TTCGGCGGTTCTCGAAAAA	50.0	47.4	57.9	58.0	359-365 bp	1.0	72.6
psbB-psbT_p2	CCAACGACAAGAAGACAGGT	TTCGGCGGTTCTCGAAAAA	50.0	47.4	57.8	58.0	296-302 bp	1.0	71.3
psbB-psbT_p3	CCGGATTTGGATGCTCAAGT	TTCGGCGGTTCTCGAAAAA	50.0	47.4	58.2	58.0	347-353 bp	1.0	71.1
psbB-psbT_p4	TGGTATTGACCCGGATTTGG	TTCGGCGGTTCTCGAAAAA	50.0	47.4	57.6	58.0	357-363 bp	1.0	69.6
psbB-psbT_p5	TTTTGCTGGTATTGACCCGG	TTCGGCGGTTCTCGAAAAA	50.0	47.4	58.5	58.0	363-369 bp	1.0	69.3