Hotspot

rps12-clpP1

LSC · 71554-71740 · 187 bp

Back to Driessenia

Pi 0.0517

MarkerSeek score 80.00

Primer available yes

Label rps12-clpP1

Feature Metadata

label_name: rps12-clpP1
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 14
pi: 0.0517
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP1_p1	TGACATTTCGGATTGGCTGT	ACAAAGAACGGACAAACCCT	45.0	45.0	57.8	57.6	346-357 bp	1.0	82.1
rps12-clpP1_p2	TGACATTTCGGATTGGCTGT	TTATGTCAGCAACGGAAGCC	45.0	50.0	57.8	58.6	290-301 bp	1.0	81.8
rps12-clpP1_p3	TTTCGGATTGGCTGTCTTGT	ACAAAGAACGGACAAACCCT	45.0	45.0	57.7	57.6	341-352 bp	1.0	81.6
rps12-clpP1_p4	TTTCGGATTGGCTGTCTTGT	TTATGTCAGCAACGGAAGCC	45.0	50.0	57.7	58.6	285-296 bp	1.0	81.3
rps12-clpP1_p5	TTCGGATTGGCTGTCTTGTG	ACAAAGAACGGACAAACCCT	50.0	45.0	58.5	57.6	340-351 bp	1.0	80.0