Hotspot

trnW-CCA-trnP-UGG

LSC · 67778-67970 · 193 bp

Back to Driessenia

Pi 0.0227

MarkerSeek score 64.80

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 6
pi: 0.0227
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	AAGAAAGGGATGTAGCGCAG	50.0	50.0	58.0	58.0	311-313 bp	1.0	81.5
trnW-CCA-trnP-UGG_p2	TTTTGGAGACCCACGTTCTG	AAGAAAGGGATGTAGCGCAG	50.0	50.0	58.0	58.0	310-312 bp	1.0	81.5
trnW-CCA-trnP-UGG_p3	TGCCGAACTGAACTAAGAGC	AAGAAAGGGATGTAGCGCAG	50.0	50.0	57.9	58.0	292-294 bp	1.0	81.3
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	271-273 bp	1.0	81.3
trnW-CCA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	AAGAAAGGGATGTAGCGCAG	50.0	50.0	57.9	58.0	325-327 bp	1.0	81.0