Hotspot

petA-psbJ

LSC · 64302-65201 · 900 bp

Back to Driessenia

Pi 0.0359

MarkerSeek score 82.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 48
pi: 0.0359
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.6	58.1	1040-1052 bp	1.0	80.2
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	GTTCATACTCAGGGTTGGGC	45.0	55.0	57.6	58.2	1044-1056 bp	1.0	79.0
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	TTCATACTCAGGGTTGGGCT	45.0	50.0	57.6	58.3	1043-1055 bp	1.0	78.4
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	GGTTCATACTCAGGGTTGGG	45.0	55.0	57.6	57.6	1045-1057 bp	1.0	77.7
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	ATACTCAGGGTTGGGCTCAT	50.0	50.0	57.2	58.1	1046-1058 bp	1.0	77.5