Hotspot

psbZ-trnG-UCC

LSC · 37314-37628 · 315 bp

Back to Driessenia

Pi 0.0341

MarkerSeek score 65.30

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 315
variable_sites: 16
pi: 0.0341
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGCTGGTCAAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	459-465 bp	1.0	80.8
psbZ-trnG-UCC_p2	CCCGTTGTTTTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	480-486 bp	1.0	80.6
psbZ-trnG-UCC_p3	ACGGCTGGTCAAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.7	57.8	458-464 bp	1.0	79.1
psbZ-trnG-UCC_p4	GTTTTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.3	57.8	474-480 bp	1.0	76.2
psbZ-trnG-UCC_p5	TTTTTGCTTCTCCTGACGGC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.0	57.8	473-479 bp	1.0	73.3