Hotspot

trnE-UUC-trnT-GGU

LSC · 31694-32646 · 953 bp

Back to Driessenia

Pi 0.023

MarkerSeek score 64.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 32
pi: 0.023
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGGATCAGGGAATTCTTGGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1240-1281 bp	1.0	84.8
trnE-UUC-trnT-GGU_p2	TCATTTCATGGCGGATCAGG	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1251-1292 bp	1.0	84.8
trnE-UUC-trnT-GGU_p3	CGGATCAGGGAATTCTTGGG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	1219-1260 bp	1.0	84.4
trnE-UUC-trnT-GGU_p4	TCATTTCATGGCGGATCAGG	CCATGGCGTTACTCTACCAC	50.0	55.0	58.0	58.1	1230-1271 bp	1.0	84.4
trnE-UUC-trnT-GGU_p5	CGGATCAGGGAATTCTTGGG	TAAACCCTTCTCTGCGAACG	55.0	50.0	58.0	57.9	1677-1731 bp	1.0	83.9