Hotspot

atpH-atpI

LSC · 13875-14831 · 957 bp

Back to Driessenia

Pi 0.0201

MarkerSeek score 61.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 957
variable_sites: 28
pi: 0.0201
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GAAGCAAGTCCGACAGCTAA	CCATGGAGGGTCATCATTGA	50.0	50.0	57.9	57.0	1039-1069 bp	1.0	75.3
atpH-atpI_p2	GAAGCAAGTCCGACAGCTAA	TCCATGGAGGGTCATCATTG	50.0	50.0	57.9	57.0	1040-1070 bp	1.0	75.3
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	CCATGGAGGGTCATCATTGA	45.0	50.0	58.1	57.0	1007-1037 bp	1.0	75.3
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	TCCATGGAGGGTCATCATTG	45.0	50.0	58.1	57.0	1008-1038 bp	1.0	75.3
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	CCATGGAGGGTCATCATTGA	50.0	50.0	57.8	57.0	1017-1047 bp	1.0	74.7