Hotspot

rps8

LSC · 79245-79655 · 411 bp

Back to Drepanostachyum

Pi 0.00649

MarkerSeek score 48.20

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 4
pi: 0.00649
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	CGAGTACTATAGGAGGGGCG	GGAGGAACCTAGAATTAGAGTCT	60.0	43.5	58.5	56.3	456 bp	1.0	40.7
rps8_p2	CGAGTACTATAGGAGGGGCG	GGAGGAACCTAGAATTAGAGTCTT	60.0	41.7	58.5	57.0	456 bp	1.0	40.7
rps8_p3	GAGTACTATAGGAGGGGCGA	GGAGGAACCTAGAATTAGAGTCT	55.0	43.5	57.1	56.3	455 bp	1.0	40.7
rps8_p4	TCGAGTACTATAGGAGGGGC	GGAGGAACCTAGAATTAGAGTCT	55.0	43.5	57.1	56.3	457 bp	1.0	40.7
rps8_p5	CGAGTACTATAGGAGGGGCG	GGAGGAACCTAGAATTAGAGTC	60.0	45.5	58.5	54.8	456 bp	1.0	40.7