Hotspot

rpoC2

LSC · 27174-31727 · 4554 bp

Back to Drepanostachyum

Pi 0.00161

MarkerSeek score 43.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4554
variable_sites: 11
pi: 0.00161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGGCACCTTGTTGAATCGAA	ACTTTTGGAAGACCTTGCGT	45.0	45.0	58.0	57.9	654 bp	1.0	80.1
rpoC2_p2	TTGGCACCTTGTTGAATCGA	ACTTTTGGAAGACCTTGCGT	45.0	45.0	58.0	57.9	655 bp	1.0	80.1
rpoC2_p3	TGGCACCTTGTTGAATCGAA	GGAAACCCGCACTTTTGATG	45.0	50.0	58.0	57.9	913 bp	1.0	79.9
rpoC2_p4	TTGGCACCTTGTTGAATCGA	GGAAACCCGCACTTTTGATG	45.0	50.0	58.0	57.9	914 bp	1.0	79.9
rpoC2_p5	TGGCACCTTGTTGAATCGAA	CAATCAAGAATCCCCAGGGG	45.0	55.0	58.0	57.9	773 bp	1.0	79.9