Hotspot

rps19

IRb · 85853-86131 · 279 bp

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Pi 0.00239

MarkerSeek score 41.50

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRb
length_bp: 279
variable_sites: 1
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	AGGCTTTTCACATGAATACT	AAAGGGAGGAGTAATCAGCC	35.0	50.0	52.4	56.6	330 bp	1.0	40.2
rps19_p2	AAGGCTTTTCACATGAATACT	AAAGGGAGGAGTAATCAGCC	33.3	50.0	53.4	56.6	331 bp	1.0	40.2
rps19_p3	AGGCTTTTCACATGAATACTT	AAAGGGAGGAGTAATCAGCC	33.3	50.0	53.4	56.6	330 bp	1.0	40.2
rps19_p4	AGGCTTTTCACATGAATACTTAGT	AAAGGGAGGAGTAATCAGCC	33.3	50.0	56.4	56.6	330 bp	1.0	40.2
rps19_p5	AAGGCTTTTCACATGAATACTT	AAAGGGAGGAGTAATCAGCC	31.8	50.0	54.2	56.6	331 bp	1.0	40.2