Hotspot

ndhA

SSC · 122049-124235 · 2187 bp

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Pi 0.000914

MarkerSeek score 39.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2187
variable_sites: 3
pi: 0.000914
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTCTTTCTCTTGGTTGAGA	TTGCAAATTCTTCCCCAGCT	36.4	45.0	55.5	58.0	2371 bp	1.0	44.1
ndhA_p2	TGTTTCTTTCTCTTGGTTGAGA	GTCCACCCGGTTTCATCAAT	36.4	50.0	55.5	58.2	2391 bp	1.0	42.9
ndhA_p3	ATGTTTCTTTCTCTTGGTTGAGA	TTGCAAATTCTTCCCCAGCT	34.8	45.0	56.0	58.0	2372 bp	1.0	40.4
ndhA_p4	TGTTTCTTTCTCTTGGTTGAGAT	TTGCAAATTCTTCCCCAGCT	34.8	45.0	56.0	58.0	2371 bp	1.0	40.4
ndhA_p5	TGTTTCTTTCTCTTGGTTGAG	TTGCAAATTCTTCCCCAGCT	38.1	45.0	54.0	58.0	2371 bp	1.0	40.1