Hotspot

cemA

LSC · 61256-61945 · 690 bp

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Pi 0.00193

MarkerSeek score 47.50

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 2
pi: 0.00193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TCGACGAATGAAACAGGTTC	ACACGAAGAAAGATTCTGATTTGA	45.0	33.3	56.1	56.9	784 bp	1.0	40.2
cemA_p2	AGAATCGACGAATGAAACAGGT	ACACGAAGAAAGATTCTGATTTGA	40.9	33.3	58.1	56.9	788 bp	1.0	40.2
cemA_p3	TCGACGAATGAAACAGGTTC	ACACGAAGAAAGATTCTGATTTG	45.0	34.8	56.1	55.5	784 bp	1.0	40.2
cemA_p4	ATCGACGAATGAAACAGGTTC	ACACGAAGAAAGATTCTGATTTGA	42.9	33.3	56.6	56.9	785 bp	1.0	40.2
cemA_p5	GAATCGACGAATGAAACAGGT	ACACGAAGAAAGATTCTGATTTGA	42.9	33.3	56.6	56.9	787 bp	1.0	40.2