Hotspot

accD-psaI

LSC · 58594-59457 · 864 bp

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Pi 0.0054

MarkerSeek score 49.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 864
variable_sites: 7
pi: 0.0054
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGTTTCCTTCCCTTGAA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	986 bp	1.0	80.4
accD-psaI_p2	CACGGTTTCCTTCCCTTGAA	CCGGAAAGACTAGGCCTACT	50.0	55.0	58.0	58.2	967 bp	1.0	78.6
accD-psaI_p3	TCAGCTACACGGTTTCCTTC	AAAGAAGCCATTGCGATTGC	50.0	45.0	57.5	58.0	993 bp	1.0	77.0
accD-psaI_p4	CACGGTTTCCTTCCCTTGAA	CGATTGCCGGAAAGACTAGG	50.0	55.0	58.0	58.4	973 bp	1.0	77.0
accD-psaI_p5	TACACGGTTTCCTTCCCTTG	AAAGAAGCCATTGCGATTGC	50.0	45.0	57.4	58.0	988 bp	1.0	76.1