Hotspot

ndhF

SSC · 107695-109926 · 2232 bp

Back to Dracocephalum

Pi 0.0223

MarkerSeek score 64.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 182
pi: 0.0223
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACCCAAGTGAATGGAAAGGG	CTGCCCAATAGCCTTTCCTT	50.0	50.0	57.7	57.9	2901-2924 bp	0.5	61.3
ndhF_p2	CGGAAGAAAAGGGGGATCTG	CTGCCCAATAGCCTTTCCTT	55.0	50.0	58.0	57.9	2954-2968 bp	0.416667	59.2
ndhF_p3	CGGAAGAAAAGGGGGATCTG	TGCCCAATAGCCTTTCCTTT	55.0	45.0	58.0	57.4	2953-2967 bp	0.416667	55.4
ndhF_p4	CGGAAGAAAAGGGGGATCTG	AAATGCTTTTAACGCTGCCC	55.0	45.0	58.0	57.9	2973-2982 bp	0.166667	47.6
ndhF_p5	ACCCAAGTGAATGGAAAGGG	AAATGCTTTTAACGCTGCCC	50.0	45.0	57.7	57.9	2929-2938 bp	0.166667	45.5