Hotspot

rps15-trnN-GUU

SSC · 120011-126271 · 6261 bp

Back to Dracocephalum

Pi 0.0231

MarkerSeek score 66.50

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 6261
variable_sites: 567
pi: 0.0231
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	ATAAAGAAGGGCCTTTCGGG	CGGAAGAAAAGGGGGATCTG	50.0	55.0	57.6	58.0	870-1128 bp	0.75	77.6
rps15-trnN-GUU_p2	ATAAAGAAGGGCCTTTCGGG	ACCCAAGTGAATGGAAAGGG	50.0	50.0	57.6	57.7	859-1084 bp	0.75	71.8
rps15-trnN-GUU_p3	GCCTTTCGGGTTTACATTGG	CGGAAGAAAAGGGGGATCTG	50.0	55.0	57.4	58.0	905-1118 bp	0.333333	51.1
rps15-trnN-GUU_p4	GGGCCTTTCGGGTTTACATT	CGGAAGAAAAGGGGGATCTG	50.0	55.0	58.4	58.0	907 bp	0.083333	39.4
rps15-trnN-GUU_p5	GGCCTTTCGGGTTTACATTG	CGGAAGAAAAGGGGGATCTG	50.0	55.0	57.4	58.0	906 bp	0.083333	37.8