Hotspot

trnP-UGG-psaJ

LSC · 64997-65275 · 279 bp

Back to Dracocephalum

Pi 0.0299

MarkerSeek score 76.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 279
variable_sites: 31
pi: 0.0299
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GGGCCGTGGAAAGATATGTT	50.0	50.0	58.1	57.9	361-390 bp	1.0	89.7
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GGGCCGTGGAAAGATATGTT	50.0	50.0	58.1	57.9	360-389 bp	1.0	89.7
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGGCCGTGGAAAGATATGTT	50.0	50.0	58.1	57.9	372-401 bp	1.0	89.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GGGCCGTGGAAAGATATGTT	50.0	50.0	58.1	57.9	371-400 bp	1.0	89.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GGGCCGTGGAAAGATATGTT	50.0	50.0	58.1	57.9	374-403 bp	1.0	89.4