Hotspot

trnF-GAA-ndhJ

LSC · 46715-47331 · 617 bp

Back to Dracocephalum

Pi 0.0229

MarkerSeek score 75.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 51
pi: 0.0229
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	673-780 bp	1.0	89.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	637-744 bp	1.0	89.6
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	674-781 bp	1.0	89.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	638-745 bp	1.0	89.0
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	57.9	58.0	661-768 bp	1.0	88.7