Hotspot

psaA-ycf3

LSC · 40337-41134 · 798 bp

Back to Dracocephalum

Pi 0.0331

MarkerSeek score 77.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 87
pi: 0.0331
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	CACAGGGCGCTTCGAATAA	50.0	52.6	57.9	58.2	866-921 bp	1.0	81.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCACAGGGCGCTTCGAATA	50.0	50.0	57.9	59.5	868-923 bp	1.0	78.7
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	CACAGGGCGCTTCGAATAA	50.0	52.6	58.5	58.2	819-874 bp	1.0	78.2
psaA-ycf3_p4	GGCCCATTCCTCGAAAGAAG	CACAGGGCGCTTCGAATAA	55.0	52.6	58.6	58.2	867-922 bp	1.0	77.0
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TCACAGGGCGCTTCGAATAA	50.0	50.0	57.9	59.8	867-922 bp	1.0	76.9