Hotspot

psbZ-trnG-GCC

LSC · 34688-34954 · 267 bp

Back to Dracocephalum

Pi 0.0479
MarkerSeek score 77.80
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
267
variable_sites
61
pi
0.0479
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 GGGTTAGTCTTTCTGGTGGG TACCATTCGACCATATCCGC 55.0 50.0 57.5 57.3 255-375 bp 1.0 82.2
psbZ-trnG-GCC_p2 GGGTTAGTCTTTCTGGTGGG TTACCATTCGACCATATCCGC 55.0 47.6 57.5 58.0 256-376 bp 1.0 79.8
psbZ-trnG-GCC_p3 AGTCTTTCTGGTGGGTATCCT TACCATTCGACCATATCCGC 47.6 50.0 57.8 57.3 250-370 bp 1.0 76.5
psbZ-trnG-GCC_p4 TGGGTTAGTCTTTCTGGTGG TACCATTCGACCATATCCGC 50.0 50.0 56.8 57.3 256-376 bp 1.0 76.2
psbZ-trnG-GCC_p5 GGTTAGTCTTTCTGGTGGGT TACCATTCGACCATATCCGC 50.0 50.0 56.8 57.3 254-374 bp 1.0 76.1