Hotspot

trnS-UGA-psbZ

LSC · 34165-34498 · 334 bp

Back to Dracocephalum

Pi 0.0285
MarkerSeek score 67.50
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
334
variable_sites
36
pi
0.0285
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAACCACTCAGCCATCTCTC AATCCTGTCTCCGCAACATC 55.0 50.0 58.0 58.0 986-1125 bp 1.0 91.6
trnS-UGA-psbZ_p2 CAACCACTCAGCCATCTCTC CTTGAGGTCACGGGTTCAAA 55.0 50.0 58.0 58.0 1004-1143 bp 1.0 91.5
trnS-UGA-psbZ_p3 TTCTGTGGGTGGTGTAGCTA AATCCTGTCTCCGCAACATC 50.0 50.0 58.0 58.0 1520-1654 bp 1.0 91.2
trnS-UGA-psbZ_p4 TTCTGTGGGTGGTGTAGCTA CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.0 58.0 1538-1672 bp 1.0 91.0
trnS-UGA-psbZ_p5 TTCTGTGGGTGGTGTAGCTA AATCGAACCCGCATCTTCTC 50.0 50.0 58.0 58.1 1325-1452 bp 1.0 91.0