Hotspot

trnG-UCC-trnR-UCU

LSC · 9422-9606 · 185 bp

Back to Dracocephalum

Pi 0.0419

MarkerSeek score 74.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 31
pi: 0.0419
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	259-279 bp	1.0	75.3
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	262-282 bp	1.0	75.1
trnG-UCC-trnR-UCU_p3	GCTAACGATGTGGGTTCGAT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.1	56.9	249-269 bp	1.0	75.1
trnG-UCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	265-285 bp	1.0	74.7
trnG-UCC-trnR-UCU_p5	TTCCAAGCTAACGATGTGGG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.9	56.9	260 bp	0.083333	26.1