Hotspot

rps16-trnQ-UUG

LSC · 5771-6682 · 912 bp

Back to Dracocephalum

Pi 0.0513
MarkerSeek score 88.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
912
variable_sites
164
pi
0.0513
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
88.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTATCCTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 974-1113 bp 1.0 91.3
rps16-trnQ-UUG_p2 GTATCCTTCAAGTCGCACGT GTCTCGCTATTCGGAGGTTC 50.0 55.0 58.0 57.9 987-1126 bp 1.0 91.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 965-1104 bp 1.0 90.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTCTCGCTATTCGGAGGTTC 45.0 55.0 58.1 57.9 978-1117 bp 1.0 89.9
rps16-trnQ-UUG_p5 GTATCCTTCAAGTCGCACGT CGGGTTTTGGTCTCGCTATT 50.0 50.0 58.0 58.3 996-1135 bp 1.0 89.8