Hotspot

ycf1

SSC · 124239-129698 · 5460 bp

Back to Dracaena

Pi 0.012

MarkerSeek score 50.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5460
variable_sites: 288
pi: 0.012
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTGCTCCCGTGTAATCTTC	CCCGTGGATCATACCAATCA	50.0	50.0	57.0	57.1	248-250 bp	0.909091	67.0
ycf1_p2	TCTGCTCCCGTGTAATCTTC	ACCCGTGGATCATACCAATC	50.0	50.0	57.0	57.1	249-251 bp	0.909091	67.0
ycf1_p3	CTGCTCCCGTGTAATCTTCT	CCCGTGGATCATACCAATCA	50.0	50.0	57.0	57.1	247-249 bp	0.909091	67.0
ycf1_p4	CTGCTCCCGTGTAATCTTCT	ACCCGTGGATCATACCAATC	50.0	50.0	57.0	57.1	248-250 bp	0.909091	66.9
ycf1_p5	TCTGCTCCCGTGTAATCTTC	CCCGTGGATCATACCAATCAA	50.0	47.6	57.0	57.8	248-250 bp	0.909091	64.8