Hotspot

ccsA-ndhD

SSC · 115700-115922 · 223 bp

Back to Dracaena

Pi 0.0351

MarkerSeek score 68.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 223
variable_sites: 22
pi: 0.0351
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGAATTCGTCACTTGTAGCG	TCAGTTGACAAGGTACAAGCT	50.0	42.9	57.3	57.2	390-398 bp	1.0	65.2
ccsA-ndhD_p2	CGAATTCGTCACTTGTAGCG	TCGCTATCAGTTGACAAGGT	50.0	45.0	57.3	56.6	396-397 bp	0.909091	63.8
ccsA-ndhD_p3	CGAATTCGTCACTTGTAGCG	TTCGCTATCAGTTGACAAGGT	50.0	42.9	57.3	57.3	397-398 bp	0.909091	61.7
ccsA-ndhD_p4	ATTCGTCACTTGTAGCGTCT	TCGCTATCAGTTGACAAGGT	45.0	45.0	57.0	56.6	393-394 bp	0.909091	61.2
ccsA-ndhD_p5	CGAATTCGTCACTTGTAGCG	TTTCGCTATCAGTTGACAAGGT	50.0	40.9	57.3	58.0	398-399 bp	0.909091	59.1