Hotspot

rpl32-trnL-UAG

SSC · 113667-114569 · 903 bp

Back to Dracaena

Pi 0.0161
MarkerSeek score 65.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
903
variable_sites
52
pi
0.0161
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAATAAGA AGCGTGTCTACCGATTTCAC 35.0 50.0 53.8 58.0 903-979 bp 1.0 54.6
rpl32-trnL-UAG_p2 TGTGCGACAAACAAATAAGA CAGCGTGTCTACCGATTTCA 35.0 50.0 53.8 58.0 904-980 bp 1.0 54.5
rpl32-trnL-UAG_p3 TGTGCGACAAACAAATAAGA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.8 57.9 913-989 bp 1.0 53.6
rpl32-trnL-UAG_p4 TGTGCGACAAACAAATAAGAA AGCGTGTCTACCGATTTCAC 33.3 50.0 54.6 58.0 903-979 bp 1.0 53.5
rpl32-trnL-UAG_p5 TTGTGCGACAAACAAATAAGA AGCGTGTCTACCGATTTCAC 33.3 50.0 54.6 58.0 904-980 bp 1.0 53.5