Hotspot

psaJ-rpl33

LSC · 67291-67671 · 381 bp

Back to Dracaena

Pi 0.0126

MarkerSeek score 62.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 16
pi: 0.0126
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	TAGAAATGCCTGGCGATTCC	50.0	50.0	58.1	58.0	554-711 bp	1.0	85.7
psaJ-rpl33_p2	ATGGTTTGGGTCTTTAGCGG	TAGAAATGCCTGGCGATTCC	50.0	50.0	58.2	58.0	559-716 bp	1.0	84.8
psaJ-rpl33_p3	TGGGTCTTTAGCGGGTCTAT	TAGAAATGCCTGGCGATTCC	50.0	50.0	57.8	58.0	553-710 bp	1.0	84.8
psaJ-rpl33_p4	TTGGGTCTTTAGCGGGTCTA	CCTGGCGATTCCTTTTCCTT	50.0	50.0	58.1	58.2	546-703 bp	1.0	84.7
psaJ-rpl33_p5	ATGGTTTGGGTCTTTAGCGG	CCTGGCGATTCCTTTTCCTT	50.0	50.0	58.2	58.2	551-708 bp	1.0	83.8