Hotspot

trnP-UGG-psaJ

LSC · 66792-67152 · 361 bp

Back to Dracaena

Pi 0.00923
MarkerSeek score 61.20
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
361
variable_sites
12
pi
0.00923
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC AGGCGCTGTGGAAAGATATG 50.0 50.0 58.1 58.0 435-453 bp 1.0 84.6
trnP-UGG-psaJ_p2 ACCCGTGACATTTTGTACCC CAGGCGCTGTGGAAAGATAT 50.0 50.0 58.1 58.0 436-454 bp 1.0 84.6
trnP-UGG-psaJ_p3 CCCGTGACATTTTGTACCCA AGGCGCTGTGGAAAGATATG 50.0 50.0 58.1 58.0 434-452 bp 1.0 84.6
trnP-UGG-psaJ_p4 CCCGTGACATTTTGTACCCA CAGGCGCTGTGGAAAGATAT 50.0 50.0 58.1 58.0 435-453 bp 1.0 84.6
trnP-UGG-psaJ_p5 ACAGGATTTGAACCCGTGAC AGGCGCTGTGGAAAGATATG 50.0 50.0 58.1 58.0 446-464 bp 1.0 84.4