Hotspot

petG-trnW-CCA

LSC · 66346-66460 · 115 bp

Back to Dracaena

Pi 0.105

MarkerSeek score 78.10

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 39
pi: 0.105
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAATACAGACGCGGTGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	156-226 bp	1.0	91.3
petG-trnW-CCA_p2	CAATACAGACGCGGTGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	155-225 bp	1.0	91.2
petG-trnW-CCA_p3	ACAATACAGACGCGGTGATC	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	120-190 bp	1.0	90.7
petG-trnW-CCA_p4	CAATACAGACGCGGTGATCA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	119-189 bp	1.0	90.7
petG-trnW-CCA_p5	CGGTGATCAGTTGGACCTTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	144-214 bp	1.0	90.4