Hotspot

accD-psaI

LSC · 59217-59600 · 384 bp

Back to Dracaena

Pi 0.0149

MarkerSeek score 61.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 15
pi: 0.0149
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GATCCAATCGTACCGCGTAA	AAAGAAGCCATTGCAATCGC	50.0	45.0	57.9	58.0	566-581 bp	1.0	82.6
accD-psaI_p2	TCCAATCGTACCGCGTAATC	AAAGAAGCCATTGCAATCGC	50.0	45.0	57.9	58.0	564-579 bp	1.0	82.6
accD-psaI_p3	CCAATCGTACCGCGTAATCT	AAAGAAGCCATTGCAATCGC	50.0	45.0	57.9	58.0	563-578 bp	1.0	82.6
accD-psaI_p4	GATCCAATCGTACCGCGTAA	GCAGGAAATACTAGGCCCAC	50.0	55.0	57.9	58.0	548-563 bp	1.0	82.5
accD-psaI_p5	TCCAATCGTACCGCGTAATC	GCAGGAAATACTAGGCCCAC	50.0	55.0	57.9	58.0	546-561 bp	1.0	82.5